pcDNA3.1(-)/myc-His C载体质粒图谱、序列、说明书、价格

产品具体信息请参见:http://www.honorgene.com/product_list/carrier_library/123250.html

如需订购此产品,请联系HonorGene客服或技术支持(www.98z.net)。

别名:pcDNA3.1/myc-His(-) C

质粒类型:哺乳动物表达载体

启动子:CMV

克隆方法:多克隆位点,限制性内切酶

载体大小:5521 bp

5' 测序引物及序列:T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'

载体标签:His Tag(6x),c-Myc Epitope Tag

载体抗性:Ampicillin (氨苄青霉素)

筛选标记:G418,neo

价格:询价

载体描述:

最常用的哺乳动物表达载体之一。

CMV promoter强启动子调控外源基因表达,C端携带myc标签及His标签,方便进行表达检测。

载体拷贝数很高,表达量也很高。

无荧光标记。

Amp+原核筛选抗性,Neo+真核筛选抗性,可以利用G418筛选稳定细胞株。

pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fr

请访问以下页面下载【pcDNA3.1(-)/myc-His C】产品说明书及载体序列:http://www.honorgene.com/product_list/carrier_library/123250.html

质粒图谱:

主营产品:破碎机,分选、筛选机械,磁选机械,重选机械,煤炭洗选设备,回转滚筒干燥设备